                         SEQUENCE LISTING

<110>  中国农业科学院生物技术研究所
 
<120>  一种重组酶介导的恒温核酸扩增技术中DNA聚合酶

<130>  B002

<160>  15    

<170>  PatentIn version 3.3

<210>  1
<211>  2838
<212>  DNA
<213>  Artificial

<220>
<223>  Sau-TBD片段


<220>
<221>  CDS
<222>  (1)..(2838)

<400>  1
gtg aat aaa tta gta tta atc gat ggt aat agc tta agt ttt aga gct       48
Val Asn Lys Leu Val Leu Ile Asp Gly Asn Ser Leu Ser Phe Arg Ala         
1               5                   10                  15              

ttt tat gca tta cct ttg tta tca aac aaa gca ggc att cat acc aat       96
Phe Tyr Ala Leu Pro Leu Leu Ser Asn Lys Ala Gly Ile His Thr Asn         
            20                  25                  30                  

gca gta tat ggt ttt gca atg tta cta gag aag att tta aaa gaa gaa      144
Ala Val Tyr Gly Phe Ala Met Leu Leu Glu Lys Ile Leu Lys Glu Glu         
        35                  40                  45                      

aag cca aat cat ttt tta gta gcg ttt gat gca ggt aaa acg act ttc      192
Lys Pro Asn His Phe Leu Val Ala Phe Asp Ala Gly Lys Thr Thr Phe         
    50                  55                  60                          

aga cat gaa aaa tat agt gaa tat aaa ggt gga cgc cag aaa acg ccg      240
Arg His Glu Lys Tyr Ser Glu Tyr Lys Gly Gly Arg Gln Lys Thr Pro         
65                  70                  75                  80          

cct gaa cta agt gag caa ttt cct tat ata cgc caa tta tta gat gca      288
Pro Glu Leu Ser Glu Gln Phe Pro Tyr Ile Arg Gln Leu Leu Asp Ala         
                85                  90                  95              

tat cat att aaa cgt tat gaa tta gat aac tat gag gca gat gat att      336
Tyr His Ile Lys Arg Tyr Glu Leu Asp Asn Tyr Glu Ala Asp Asp Ile         
            100                 105                 110                 

atc gga act tta agt aaa gaa gca gat aaa gcg ggc ttt cag aca att      384
Ile Gly Thr Leu Ser Lys Glu Ala Asp Lys Ala Gly Phe Gln Thr Ile         
        115                 120                 125                     

att att acg gga gac cga gat tta aca caa ctt gca acg gat aat gta      432
Ile Ile Thr Gly Asp Arg Asp Leu Thr Gln Leu Ala Thr Asp Asn Val         
    130                 135                 140                         

acc att tat tac act aaa aaa ggt gtt act gat gtt gat cat tat aca      480
Thr Ile Tyr Tyr Thr Lys Lys Gly Val Thr Asp Val Asp His Tyr Thr         
145                 150                 155                 160         

cct gac ttt att gcg gaa aaa tac aac ggg tta aca cct aat caa att      528
Pro Asp Phe Ile Ala Glu Lys Tyr Asn Gly Leu Thr Pro Asn Gln Ile         
                165                 170                 175             

att gat atg aaa gga tta atg gga gat act tct gat aat ata cca ggt      576
Ile Asp Met Lys Gly Leu Met Gly Asp Thr Ser Asp Asn Ile Pro Gly         
            180                 185                 190                 

gtt gca ggt gtt gga gag aaa aca gca ata aaa ttg cta aac caa ttt      624
Val Ala Gly Val Gly Glu Lys Thr Ala Ile Lys Leu Leu Asn Gln Phe         
        195                 200                 205                     

gac aca gta gaa ggt gtc tat gaa cat tta gat gaa att tca ggt aaa      672
Asp Thr Val Glu Gly Val Tyr Glu His Leu Asp Glu Ile Ser Gly Lys         
    210                 215                 220                         

aag tta aaa gag aaa ctt caa aat agc aaa gaa gat gca tta atg agt      720
Lys Leu Lys Glu Lys Leu Gln Asn Ser Lys Glu Asp Ala Leu Met Ser         
225                 230                 235                 240         

aaa gaa tta gca acg att aat gta gat agt ccg att gaa gta aaa ctt      768
Lys Glu Leu Ala Thr Ile Asn Val Asp Ser Pro Ile Glu Val Lys Leu         
                245                 250                 255             

gaa gat acg tta atg act cat caa gat gaa caa caa gaa aaa atc gaa      816
Glu Asp Thr Leu Met Thr His Gln Asp Glu Gln Gln Glu Lys Ile Glu         
            260                 265                 270                 

ttg ttt aag aag tta gaa ttc aaa caa ttg ttg gct gac att gat caa      864
Leu Phe Lys Lys Leu Glu Phe Lys Gln Leu Leu Ala Asp Ile Asp Gln         
        275                 280                 285                     

tca gca agc gtt gaa gat gca ata gaa aag aca att gaa att gaa acg      912
Ser Ala Ser Val Glu Asp Ala Ile Glu Lys Thr Ile Glu Ile Glu Thr         
    290                 295                 300                         

tct ttt gat aat gtt gat ttt act tca ttg aaa gaa gcg gcc atc cat      960
Ser Phe Asp Asn Val Asp Phe Thr Ser Leu Lys Glu Ala Ala Ile His         
305                 310                 315                 320         

ttc gaa tta gac ggt ggt aac tat ttg cga aat aat att tta aaa ttc     1008
Phe Glu Leu Asp Gly Gly Asn Tyr Leu Arg Asn Asn Ile Leu Lys Phe         
                325                 330                 335             

tct tta ttt aca ggt gag aaa cat att gta att aat gcg gat gac ata     1056
Ser Leu Phe Thr Gly Glu Lys His Ile Val Ile Asn Ala Asp Asp Ile         
            340                 345                 350                 

aat aat tat gcc gaa cta gtt tca tgg tta gaa aat ccg aat acg aaa     1104
Asn Asn Tyr Ala Glu Leu Val Ser Trp Leu Glu Asn Pro Asn Thr Lys         
        355                 360                 365                     

aaa gtc gta tat gat gct aaa aaa aca tat gta gca tca cat aga ttg     1152
Lys Val Val Tyr Asp Ala Lys Lys Thr Tyr Val Ala Ser His Arg Leu         
    370                 375                 380                         

gga att gat att caa aat att tct ttc gat att atg ttg gcc agt tat     1200
Gly Ile Asp Ile Gln Asn Ile Ser Phe Asp Ile Met Leu Ala Ser Tyr         
385                 390                 395                 400         

ata att gat cca tct cgt acg att agt gat gtt caa tca gtt gtt tca     1248
Ile Ile Asp Pro Ser Arg Thr Ile Ser Asp Val Gln Ser Val Val Ser         
                405                 410                 415             

ttg tac ggt caa agt ttt gtg aaa gac gat gtg agt ata tat gga aaa     1296
Leu Tyr Gly Gln Ser Phe Val Lys Asp Asp Val Ser Ile Tyr Gly Lys         
            420                 425                 430                 

ggt aag aaa ttt aag gta cct gaa gat gat gtt tta aat cca tat gtt     1344
Gly Lys Lys Phe Lys Val Pro Glu Asp Asp Val Leu Asn Pro Tyr Val         
        435                 440                 445                     

gct tct att act gat gca att tac ttt gcg aaa cca aat atg gat aaa     1392
Ala Ser Ile Thr Asp Ala Ile Tyr Phe Ala Lys Pro Asn Met Asp Lys         
    450                 455                 460                         

caa tta gaa gaa tac aat cag gta gaa ctc tta gct gat tta gag cta     1440
Gln Leu Glu Glu Tyr Asn Gln Val Glu Leu Leu Ala Asp Leu Glu Leu         
465                 470                 475                 480         

ccg cta gct aag att tta agt gaa atg gaa gaa att ggt ata ttt aca     1488
Pro Leu Ala Lys Ile Leu Ser Glu Met Glu Glu Ile Gly Ile Phe Thr         
                485                 490                 495             

gat gtt cat gat tta gaa gaa atg gaa aaa gaa att caa gaa aaa tta     1536
Asp Val His Asp Leu Glu Glu Met Glu Lys Glu Ile Gln Glu Lys Leu         
            500                 505                 510                 

gac gtc ttg att cga aat atc cat gat gca gct ggt tcg tgg tat cag     1584
Asp Val Leu Ile Arg Asn Ile His Asp Ala Ala Gly Ser Trp Tyr Gln         
        515                 520                 525                     

cct aaa ggt ggc act gag atg ttc tgc cat ccg cga aca ggt aag cca     1632
Pro Lys Gly Gly Thr Glu Met Phe Cys His Pro Arg Thr Gly Lys Pro         
    530                 535                 540                         

cta cct aaa tac cct cgc att aag aca cct aaa gtt ggt ggt atc ttt     1680
Leu Pro Lys Tyr Pro Arg Ile Lys Thr Pro Lys Val Gly Gly Ile Phe         
545                 550                 555                 560         

aag aag cct aag aac aag gca cag cga gaa ggc cgt gag cct tgc gaa     1728
Lys Lys Pro Lys Asn Lys Ala Gln Arg Glu Gly Arg Glu Pro Cys Glu         
                565                 570                 575             

ctt gat acc cgc gag tac gtt gct ggt gct cct tac acc cca gtt gaa     1776
Leu Asp Thr Arg Glu Tyr Val Ala Gly Ala Pro Tyr Thr Pro Val Glu         
            580                 585                 590                 

cat gtt gtg ttt aac cct tcg tct cct aag caa tta ggt gtt gta ttg     1824
His Val Val Phe Asn Pro Ser Ser Pro Lys Gln Leu Gly Val Val Leu         
        595                 600                 605                     

ttt gag aca tta caa tta cct gtt att aaa aag acg aaa aca gga tat     1872
Phe Glu Thr Leu Gln Leu Pro Val Ile Lys Lys Thr Lys Thr Gly Tyr         
    610                 615                 620                         

tct act gct gta gat gtc tta gag caa ttg caa ggt gaa cat cct atc     1920
Ser Thr Ala Val Asp Val Leu Glu Gln Leu Gln Gly Glu His Pro Ile         
625                 630                 635                 640         

att gat tat att tta gaa tat cgt caa tta tct aag tta cag tct act     1968
Ile Asp Tyr Ile Leu Glu Tyr Arg Gln Leu Ser Lys Leu Gln Ser Thr         
                645                 650                 655             

tat gtt gaa gga ttg caa aag gta att agt gat gat caa cgt atc cat     2016
Tyr Val Glu Gly Leu Gln Lys Val Ile Ser Asp Asp Gln Arg Ile His         
            660                 665                 670                 

aca cgt ttt aac caa act tta gcg caa act gga cgt tta tca agt gta     2064
Thr Arg Phe Asn Gln Thr Leu Ala Gln Thr Gly Arg Leu Ser Ser Val         
        675                 680                 685                     

gat cct aat tta caa aat att ccg gtt aga ctt gaa gaa ggg cgt aaa     2112
Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Leu Glu Glu Gly Arg Lys         
    690                 695                 700                         

ata aga aaa gcc ttt aaa cca act tca aaa gat agc gtt ata tta tca     2160
Ile Arg Lys Ala Phe Lys Pro Thr Ser Lys Asp Ser Val Ile Leu Ser         
705                 710                 715                 720         

gca gat tat tct caa att gaa ttg cgt gta tta gca cac att aca caa     2208
Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Ile Thr Gln         
                725                 730                 735             

gat gag agt atg aaa gaa gca ttt atc aac ggc gat gat att cat aca     2256
Asp Glu Ser Met Lys Glu Ala Phe Ile Asn Gly Asp Asp Ile His Thr         
            740                 745                 750                 

gca act gct atg aaa gta ttt ggt gta gaa gct gat caa gtc gat agt     2304
Ala Thr Ala Met Lys Val Phe Gly Val Glu Ala Asp Gln Val Asp Ser         
        755                 760                 765                     

tta atg cgt cgt caa gca aaa gcg gtt aac ttt gga att gtt tat ggg     2352
Leu Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly         
    770                 775                 780                         

ata agt gat tat ggt tta agt caa agt tta ggt att act cgt aaa aaa     2400
Ile Ser Asp Tyr Gly Leu Ser Gln Ser Leu Gly Ile Thr Arg Lys Lys         
785                 790                 795                 800         

gca aaa gca ttc att gat gat tat tta gct agt ttc cca ggt gta aaa     2448
Ala Lys Ala Phe Ile Asp Asp Tyr Leu Ala Ser Phe Pro Gly Val Lys         
                805                 810                 815             

caa tat atg tct gat att gta aaa gat gcc aaa gct tta ggt tat gtg     2496
Gln Tyr Met Ser Asp Ile Val Lys Asp Ala Lys Ala Leu Gly Tyr Val         
            820                 825                 830                 

gaa aca ttg cta cat cgt cga cgc tat att cct gat att acg agt cgt     2544
Glu Thr Leu Leu His Arg Arg Arg Tyr Ile Pro Asp Ile Thr Ser Arg         
        835                 840                 845                     

aac ttt aat tta cgc ggc ttt gct gaa cgt act gct atg aat acg cca     2592
Asn Phe Asn Leu Arg Gly Phe Ala Glu Arg Thr Ala Met Asn Thr Pro         
    850                 855                 860                         

ata caa ggc agt gct gca gat atc att aaa ctg gca atg gtt aaa ttt     2640
Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Leu Ala Met Val Lys Phe         
865                 870                 875                 880         

gca caa aaa atg aaa gag aca aca tat caa gct aaa cta tta tta caa     2688
Ala Gln Lys Met Lys Glu Thr Thr Tyr Gln Ala Lys Leu Leu Leu Gln         
                885                 890                 895             

gta cac gat gaa tta att ttt gaa gta cct aag tca gaa gta gat tca     2736
Val His Asp Glu Leu Ile Phe Glu Val Pro Lys Ser Glu Val Asp Ser         
            900                 905                 910                 

ttt agt gaa ttt gta gaa gag ata atg gaa aat gca ttg caa tta gat     2784
Phe Ser Glu Phe Val Glu Glu Ile Met Glu Asn Ala Leu Gln Leu Asp         
        915                 920                 925                     

gtt cca tta aaa gta gac tca agt tat ggt gca act tgg tat gat gca     2832
Val Pro Leu Lys Val Asp Ser Ser Tyr Gly Ala Thr Trp Tyr Asp Ala         
    930                 935                 940                         

aaa taa                                                             2838
Lys                                                                     
945                                                                     


<210>  2
<211>  945
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  2

Val Asn Lys Leu Val Leu Ile Asp Gly Asn Ser Leu Ser Phe Arg Ala 
1               5                   10                  15      


Phe Tyr Ala Leu Pro Leu Leu Ser Asn Lys Ala Gly Ile His Thr Asn 
            20                  25                  30          


Ala Val Tyr Gly Phe Ala Met Leu Leu Glu Lys Ile Leu Lys Glu Glu 
        35                  40                  45              


Lys Pro Asn His Phe Leu Val Ala Phe Asp Ala Gly Lys Thr Thr Phe 
    50                  55                  60                  


Arg His Glu Lys Tyr Ser Glu Tyr Lys Gly Gly Arg Gln Lys Thr Pro 
65                  70                  75                  80  


Pro Glu Leu Ser Glu Gln Phe Pro Tyr Ile Arg Gln Leu Leu Asp Ala 
                85                  90                  95      


Tyr His Ile Lys Arg Tyr Glu Leu Asp Asn Tyr Glu Ala Asp Asp Ile 
            100                 105                 110         


Ile Gly Thr Leu Ser Lys Glu Ala Asp Lys Ala Gly Phe Gln Thr Ile 
        115                 120                 125             


Ile Ile Thr Gly Asp Arg Asp Leu Thr Gln Leu Ala Thr Asp Asn Val 
    130                 135                 140                 


Thr Ile Tyr Tyr Thr Lys Lys Gly Val Thr Asp Val Asp His Tyr Thr 
145                 150                 155                 160 


Pro Asp Phe Ile Ala Glu Lys Tyr Asn Gly Leu Thr Pro Asn Gln Ile 
                165                 170                 175     


Ile Asp Met Lys Gly Leu Met Gly Asp Thr Ser Asp Asn Ile Pro Gly 
            180                 185                 190         


Val Ala Gly Val Gly Glu Lys Thr Ala Ile Lys Leu Leu Asn Gln Phe 
        195                 200                 205             


Asp Thr Val Glu Gly Val Tyr Glu His Leu Asp Glu Ile Ser Gly Lys 
    210                 215                 220                 


Lys Leu Lys Glu Lys Leu Gln Asn Ser Lys Glu Asp Ala Leu Met Ser 
225                 230                 235                 240 


Lys Glu Leu Ala Thr Ile Asn Val Asp Ser Pro Ile Glu Val Lys Leu 
                245                 250                 255     


Glu Asp Thr Leu Met Thr His Gln Asp Glu Gln Gln Glu Lys Ile Glu 
            260                 265                 270         


Leu Phe Lys Lys Leu Glu Phe Lys Gln Leu Leu Ala Asp Ile Asp Gln 
        275                 280                 285             


Ser Ala Ser Val Glu Asp Ala Ile Glu Lys Thr Ile Glu Ile Glu Thr 
    290                 295                 300                 


Ser Phe Asp Asn Val Asp Phe Thr Ser Leu Lys Glu Ala Ala Ile His 
305                 310                 315                 320 


Phe Glu Leu Asp Gly Gly Asn Tyr Leu Arg Asn Asn Ile Leu Lys Phe 
                325                 330                 335     


Ser Leu Phe Thr Gly Glu Lys His Ile Val Ile Asn Ala Asp Asp Ile 
            340                 345                 350         


Asn Asn Tyr Ala Glu Leu Val Ser Trp Leu Glu Asn Pro Asn Thr Lys 
        355                 360                 365             


Lys Val Val Tyr Asp Ala Lys Lys Thr Tyr Val Ala Ser His Arg Leu 
    370                 375                 380                 


Gly Ile Asp Ile Gln Asn Ile Ser Phe Asp Ile Met Leu Ala Ser Tyr 
385                 390                 395                 400 


Ile Ile Asp Pro Ser Arg Thr Ile Ser Asp Val Gln Ser Val Val Ser 
                405                 410                 415     


Leu Tyr Gly Gln Ser Phe Val Lys Asp Asp Val Ser Ile Tyr Gly Lys 
            420                 425                 430         


Gly Lys Lys Phe Lys Val Pro Glu Asp Asp Val Leu Asn Pro Tyr Val 
        435                 440                 445             


Ala Ser Ile Thr Asp Ala Ile Tyr Phe Ala Lys Pro Asn Met Asp Lys 
    450                 455                 460                 


Gln Leu Glu Glu Tyr Asn Gln Val Glu Leu Leu Ala Asp Leu Glu Leu 
465                 470                 475                 480 


Pro Leu Ala Lys Ile Leu Ser Glu Met Glu Glu Ile Gly Ile Phe Thr 
                485                 490                 495     


Asp Val His Asp Leu Glu Glu Met Glu Lys Glu Ile Gln Glu Lys Leu 
            500                 505                 510         


Asp Val Leu Ile Arg Asn Ile His Asp Ala Ala Gly Ser Trp Tyr Gln 
        515                 520                 525             


Pro Lys Gly Gly Thr Glu Met Phe Cys His Pro Arg Thr Gly Lys Pro 
    530                 535                 540                 


Leu Pro Lys Tyr Pro Arg Ile Lys Thr Pro Lys Val Gly Gly Ile Phe 
545                 550                 555                 560 


Lys Lys Pro Lys Asn Lys Ala Gln Arg Glu Gly Arg Glu Pro Cys Glu 
                565                 570                 575     


Leu Asp Thr Arg Glu Tyr Val Ala Gly Ala Pro Tyr Thr Pro Val Glu 
            580                 585                 590         


His Val Val Phe Asn Pro Ser Ser Pro Lys Gln Leu Gly Val Val Leu 
        595                 600                 605             


Phe Glu Thr Leu Gln Leu Pro Val Ile Lys Lys Thr Lys Thr Gly Tyr 
    610                 615                 620                 


Ser Thr Ala Val Asp Val Leu Glu Gln Leu Gln Gly Glu His Pro Ile 
625                 630                 635                 640 


Ile Asp Tyr Ile Leu Glu Tyr Arg Gln Leu Ser Lys Leu Gln Ser Thr 
                645                 650                 655     


Tyr Val Glu Gly Leu Gln Lys Val Ile Ser Asp Asp Gln Arg Ile His 
            660                 665                 670         


Thr Arg Phe Asn Gln Thr Leu Ala Gln Thr Gly Arg Leu Ser Ser Val 
        675                 680                 685             


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Leu Glu Glu Gly Arg Lys 
    690                 695                 700                 


Ile Arg Lys Ala Phe Lys Pro Thr Ser Lys Asp Ser Val Ile Leu Ser 
705                 710                 715                 720 


Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Ile Thr Gln 
                725                 730                 735     


Asp Glu Ser Met Lys Glu Ala Phe Ile Asn Gly Asp Asp Ile His Thr 
            740                 745                 750         


Ala Thr Ala Met Lys Val Phe Gly Val Glu Ala Asp Gln Val Asp Ser 
        755                 760                 765             


Leu Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly 
    770                 775                 780                 


Ile Ser Asp Tyr Gly Leu Ser Gln Ser Leu Gly Ile Thr Arg Lys Lys 
785                 790                 795                 800 


Ala Lys Ala Phe Ile Asp Asp Tyr Leu Ala Ser Phe Pro Gly Val Lys 
                805                 810                 815     


Gln Tyr Met Ser Asp Ile Val Lys Asp Ala Lys Ala Leu Gly Tyr Val 
            820                 825                 830         


Glu Thr Leu Leu His Arg Arg Arg Tyr Ile Pro Asp Ile Thr Ser Arg 
        835                 840                 845             


Asn Phe Asn Leu Arg Gly Phe Ala Glu Arg Thr Ala Met Asn Thr Pro 
    850                 855                 860                 


Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Leu Ala Met Val Lys Phe 
865                 870                 875                 880 


Ala Gln Lys Met Lys Glu Thr Thr Tyr Gln Ala Lys Leu Leu Leu Gln 
                885                 890                 895     


Val His Asp Glu Leu Ile Phe Glu Val Pro Lys Ser Glu Val Asp Ser 
            900                 905                 910         


Phe Ser Glu Phe Val Glu Glu Ile Met Glu Asn Ala Leu Gln Leu Asp 
        915                 920                 925             


Val Pro Leu Lys Val Asp Ser Ser Tyr Gly Ala Thr Trp Tyr Asp Ala 
    930                 935                 940                 


Lys 
945 


<210>  3
<211>  250
<212>  DNA
<213>  Artificial

<220>
<223>  TBD片段

<400>  3
attgaccgaa acgttcggct cgtggtatca gcctaaaggt ggcactgaga tgttctgcca     60

tccgcgaaca ggtaagccac tacctaaata ccctcgcatt aagacaccta aagttggtgg    120

tatctttaag aagcctaaga acaaggcaca gcgagaaggc cgtgagcctt gcgaacttga    180

tacccgcgag tacgttgctg gtgctcctta caccccagtt gaacatgttg tgtttaaccc    240

ttcgtctcgt                                                           250


<210>  4
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  扩增Sau基因中PT1片段的上游引物

<400>  4
catgccatgg aacatcatca tcatcatcat tcagcaagcg ttgaag                    46


<210>  5
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  扩增Sau基因中PT1片段的下游引物

<400>  5
gataccacga accagctgca tcatggat                                        28


<210>  6
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  扩增Sau基因中PT2片段的上游引物

<400>  6
gttgtgttta acccttcgtc tcctaagcaa ttaggtg                              37


<210>  7
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  扩增Sau基因中PT2片段的下游引物

<400>  7
cgcggatcct tattttgcat catacc                                          26


<210>  8
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  扩增TBD片段的上游引物

<400>  8
tgcagctggt tcgtggtatc agcctaaagg                                      30


<210>  9
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  扩增TBD片段的下游引物

<400>  9
cacctaattg cttaggagac gaagggttaa acacaac                              37


<210>  10
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  扩增TrxA基因的上游引物

<400>  10
catgccatgg gcagcgataa aattattcac ctg                                  33


<210>  11
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  扩增TrxA基因的下游引物

<400>  11
ttttcctttt gcggccgccg ccaggttagc gtcgagg                              37


<210>  12
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  测试引物M13-40

<400>  12
gttttcccag tcacgacg                                                   18


<210>  13
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  测试引物M13-40LF

<400>  13
gttttcccag tcacgacgtt gtaaaacgac ggcc                                 34


<210>  14
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  测试引物500_F

<400>  14
actactaaat cctgaatagc tttaagaagg                                      30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  测试引物500_R

<400>  15
cagaaagcta aatatggaaa actacaatac                                      30


